Instrumental method of Analysis BP701 MCQ UNIT 1
1 The region below 2000A° is called as
(a) Near UV region
(b) UV region
(c) Far Vacuum UV region
(d) Step up UV region
SOLUTION
Far Vacuum UV region
2 If temperature is increased, what happens to fluorescence intensity
(a) Decrease in fluorescence intensity
(b) Increase in fluorescence intensity
(c) Both a & b are correct answers
(d) None of the above
SOLUTION
Decrease in fluorescence intensity
3 The filter which absorbs UV & transmits visible radiation is
(a) Excitation filter
(b) Emission filter
(c) Primary filter
(d) Secondary filter
SOLUTION
Secondary filter
4 What is role of slit in uv-visible spectroscopy
(a) Monochromatic radiation to polychromatic radiation.
(b) Polychromatic radiation to monochromatic radiation.
(c) A and B
(d) None of this
SOLUTION
Polychromatic radiation to monochromatic radiation.
5 The UV region at 200-400nm and below 200 nm are called as
(a) Near UV & vacuum UV region respectively
(b) Vacuum UV & near UV region respectively
(c) Far UV & vacuum region respectively
(d) Far UV & near UV region respectively
SOLUTION
Near UV & vacuum UV region respectively
6 If the spectrum is transparent above 200mμ, it indicates absence of
(a) Enol
(b) Conjugation
(c) Dienes
(d) Alkyl group
SOLUTION
Conjugation
7 By increasing the polarity of solvents the n ⟶ π* n ⟶ σ* π ⟶ π* experience which type of wavelengths.
(a) Shorter, longer, shorter wave- lengths respectively
(b) Shorter, longer, longer wave- lengths respectively
(c) Shorter, shorter, longer wave- lengths respectively
(d) Longer, longer, shorter wave- lengths respectively
SOLUTION
Shorter, shorter, longer wave- lengths respectively
8 Which of the following are the examples for electron with drawing groups
(a) —NH2
(b) -NO2
(c) -OH
(d) —CH3
SOLUTION
-NO2
9 In π ⟶ π* transition in conjugated system which of the following bands will occur
(a) K band
(b) R band
(c) B band
(d) E band
SOLUTION
K band
10 Oxidation product of vitamin – B1 is
(a) Thiamine diketone
(b) Thiamine
(c) Thiochrome
(d) None of these
SOLUTION
Thiochrome
11 If the wavelength of emitted radiation is shorter than the absorbed radiation is called as
(a) Raleigh fluorescence
(b) Stokes fluorescence
(c) Anti-stokes fluorescence
(d) Resonance fluorescence
SOLUTION
Anti-stokes fluorescence
13 Calculate the λmax for the given structure.
(a) 239mμ
(b) 249mμ
(c) 259mμ
(d)269mμ
SOLUTION
239mμ
14 For UV- Visible spectrum electronic excitation occur in the range of
(a) 200-800 mμ
(b) 400-800 mμ
(c) 400-700 mμ
(d) 300-600 mμ
SOLUTION
200-800 mμ
15 Which groups reduce fluorescence intensity
Hydrogen bonding groups
(a) Electron donating groups
(b) Electro negativity groups
(c) Electron with drawing groups
(d) None of these
SOLUTION
Electron with drawing groups
16 Diffraction grating is consists of a
(a) Glass
(b) Quartz
(c) Alkyl halide
(d) All of the above
SOLUTION
All of the above
17 Out of K, B, R bands which of the following bands appears at longer wavelength in the spectrum of an aromatic compound
(a) K band
(b) R band
(c) E band
(d) B band
SOLUTION
R band
18 The region between 2000A° to 4000A° is called as
(a) Far UV region
(b) Near UV region
(c) UV region
(d) End UV region
SOLUTION
Near UV region
19 Which of the following compound is well susceptible to oxygen
(a) Naphthalene
(b) Anthracene
(c) Phenanthcene
(d) Benzene
SOLUTION
Anthracene
20 If a substance is exposed to certain different value of frequency then, what happens to energy and intensity of radiation
(a) Energy is not absorbed, loss in intensity respectively
(b) Energy is not absorbed, no loss in intensity respectively
(c) Energy is absorbed, loss in intensity respectively
(d) Energy is absorbed, no loss in intensity respectively
SOLUTION
Energy is not absorbed, no loss in intensity respectively
21 Beer’s law depends upon
(a) Intensity of incident light
(b) Concentration of the solution
(c) Intensity of incident light & radiation
(d) Intensity of incident light & concentration of the solution
SOLUTION
Intensity of incident light & concentration of the solution
22 In enones which type of bands will occur
(a) E band
(b) R band
(c) K band
(d) B band
SOLUTION
R band
23 The color yellow is produced for which type of compounds
(a) Halogens
(b) Nitro compounds
(c) Alcohols
(d) Aldehydes
SOLUTION
Nitro compounds
24 In higher energy state, if spin of electrons are paired then it is called as
(a) Excited doublet state
(b) Excited singlet state
(c) Excited quadruplet state
(d) Excited triplet state
SOLUTION
Excited singlet state
25 When carbonyl compounds are placed in a suitable solvent and by gradually increasing the polarity of the solvent, the n ⟶ π* π ⟶ π* transition undergoes
(a) Red & blue shift respectively
(b) Blue & red shift respectively
(c) Black & blue shift respectively
(d) Pink &red shift respectively
SOLUTION
Blue & red shift respectively
26 Spectrophotometer is used to detect the
(a) Refraction
(b) % Absorbance
(c) % Transmittance
(d) Diffraction
SOLUTION
% Transmittance
28 Which of the following compounds for which beer’s & lambert’s law is obeyed
(a) Tautomers
(b) Conjugated double bonds, Aromatic compounds
(c) Fluorescent compounds
(d) Complexes
SOLUTION
Conjugated double bonds, Aromatic compounds
29 If viscosity of the molecule is increased then fluorescence intensity will be
(a) No change in fluorescence intensity
(b) Decrease in fluorescence intensity
(c) Increase in fluorescence intensity
(d) None of the above
SOLUTION
Increase in fluorescence intensity
31 Fluorescence intensity is directly proportional to
(a) Concentration of substance
(b) Radiation from the sources
(c) Temperature
(d) Viscosity
SOLUTION
Concentration of substance
33 Arrange the increasing order of energy required for the following various transitions
(a)σ ⟶ σ* > n ⟶ σ* > π ⟶ π* > n ⟶ π*
n⟶ σ* < n ⟶ π* < σ ⟶ σ* > π ⟶ π*
n ⟶ π* < n ⟶ σ* < π ⟶ π* < σ ⟶ σ)*
n ⟶ π* < π ⟶ π* < n ⟶ σ* < σ ⟶ σ*
SOLUTION
n ⟶ π* < π ⟶ π* < n ⟶ σ* < σ ⟶ σ*
34 Which of the following salts can be detected in Fluorometry in the field of nuclear research
Francium
Radium
Uranium
Thallium
SOLUTION
Uranium
35 What is the other name for R band
Forbidden bands
Transition bands
Step up bands
Cyclic bands
SOLUTION
Forbidden bands
36 Increase in intensity of absorption maximum and decrease in intensity of absorption maximum are called as
Hyper & Hyper chromic shift respectively
Hypo & Hypo chromic shift respectively
Hypo & Hyper chromic shift respectively
Hyper & Hypo chromic shift respectively
SOLUTION
Hyper & Hypo chromic shift respectively
37 If a compound having a single chromophore and having at least one lone pair of electrons on hetero atom experience then which of the following bands will occur
B band
E band
R band
K band
SOLUTION
R band
38 The work of Entrance slit is
To get narrow source
To render light parallel
To disperse radiation
To fall on sample cell
SOLUTION
To get narrow source
39 If ethylene acts as chromophore then which of the following type of transition will occur
π ⟶ π*
n ⟶ π*
π ⟶ π
π* ⟶ n
SOLUTION
n ⟶ π*
40 The region below 200 mμ is also called as
Flushing UV region
Forbidden UV region
Vacuum UV region
Dispersion UV region
SOLUTION
Vacuum UV region
41Which of the following vitamin’s can be detected by Fluorometry
Vit -B3
Vit -B2
Vit -B12
Vit -B6
SOLUTION
Vit -B2
42 Calculate the λmax for
255mμ
225 mμ
245mμ
235 mμ
SOLUTION
235 mμ
44 The study of measurement of emitted radiation of electrons undergo transition from singlet excited to singlet ground state is
Phosphorescence
Flame photometry
Fluorometry
Excitation
SOLUTION
Fluorometry
45 E2 band of E- Band appears at
Longer wavelength
Shorter wavelength
Longer wave number
Shorter wave number
SOLUTION
Longer wavelength
46 Tungsten lamp filament has required how much temperature
2000k
3000k
4000k
5000k
SOLUTION
3000k
47 A record of amount of light absorbed by the sample as a function of the wavelength of light is called as
Excitation band
Adsorption band
Electronic band
Absorption band
SOLUTION
Absorption band
49 n ⟶ π* transition for α, β-unsaturated carbonyl compounds, the ground which states will be___ compared to excited state.
Non polar
More polar
Polar
Less polar
SOLUTION
More polar
50 If flexible structures are present then
Less phosphorescence intensity
More fluorescence intensity
Less fluorescence intensity
More phosphorescence intensity
SOLUTION
Less fluorescence intensity
51 If the number of double bonds increases in a compound then, the compound experiences which type of wavelength
Shorter
More shorter
Less shorter
Longer
SOLUTION
Longer
52 (a) Sharp peaks are rarely observed than broad peaks
(b) Due to vibrational & rotational effects are superimposed on the electronic transitions
A &B are wrong
A is correct, B is wrong
A & B are correct
A is wrong & B is correct
SOLUTION
A & B are correct
53 Shift in absorption maximum towards longer wavelength is called as
Bathochromic shift
Hypsochromic shift
Hyperchromic shift
Hypochromic shift
SOLUTION
Bathochromic shift
54 (a) The UV spectroscopy is called as Electronic Spectroscopy.
(b) Longer wavelength region of Visible and the
near UV region of electromagnetic radiation cause electronic transition in the molecule and produces electronic spectrum.
A & B are correct
A is correct, B is wrong
A is wrong, B is correct
A & B are incorrect
SOLUTION
A is correct, B is wrong
55 The value k = absorption coefficient depends upon
Nature of the absorbing medium
Thickness of the absorbing medium
Intensity of the absorbing medium
Radiation
SOLUTION
Nature of the absorbing medium
56 The UV region extending from 100-200 nm is called as
Vacuum region
Visible region
Near UV region
Far UV region
SOLUTION
Far UV region
57 The value a = extinction coefficient of the absorbing medium is written as
a= Io e-kx
a =1/2.303
a = k/2.303
a = Io 10-ax
SOLUTION
a = k/2.303
58 σ ⟶ σ* transition requires
Very short wavelength, high energy
Very short wavelength, low energy
Very high wavelength, high energy
Very high wavelength, low energy
SOLUTION
Very short wavelength, high energy
59 According to Born-Oppenheimer approximation, the total energy of the molecule is given by
ETotal=ETransitional+EVibrational+EElectronic
ETotal=EVibrational+ETransitional+EElectronic
ETotal= ETransitional+EVibrational+ ERotational+EElectronic
ETotal=ETransitional+ERotaiional+EElectronic
SOLUTION
ETotal= ETransitional+EVibrational+ ERotational+EElectronic
60 The materials which exhibits fluorescence intensity can emit excess radiation
10-4 -10-6 sec
10-3 – 10-2 sec
10-5- 10-3 sec
10-6-10-4 sec
SOLUTION
10-6-10-4 sec
61 Which of the following is not used as fluorescent indicator in Fluorometry
Eosin
Acridines
3-Napthoquinone
2-Napthoquinone
SOLUTION
3-Napthoquinone
62Calculate the absorption maximum in the UV spectrum of 2, 4 octadiene.
227mμ
237mμ
217mμ
247mμ
SOLUTION
237mμ
63 Which of the following halo compounds require high energy to excite
Methyl Fluoride
Methyl Iodide
Methyl Chloride
Methyl Bromide
SOLUTION
Methyl Chloride
64 In π ⟶ π* transition for α, β unsaturated carbonyl compounds the absorption band will be move to
Longer wavelength
Same wavelength
Shorter wavelength
None
SOLUTION
Shorter wavelength
65 Which type of vapour is stored in Mercury lamp
Mercury vapour
Hydrogen vapour
Xe
Ozone
SOLUTION
Mercury vapour
66 The emitted radiation in fluorescence will be
Longer
Shorter
Longer and shorter
None of the above
SOLUTION
Longer
67 Which of the following function groups will not acts as auxochrome
-OH
– CO=
– OR
– NH2
SOLUTION
– CO=
68 The conjugated polyene system appears colored to the naked eye, the number of double bonds present in those system will be
More than two
More than one
More than three
More than five
SOLUTION
More than five
69 In π ⟶ π* transition in aromatic or hetero- aromatic molecules experience which type of band
K band
B band
E band
R band
SOLUTION
B band
70 Calculate the energy associated with radiation having wavelength 280mμ
110 kcal / mol
100 kjoule / mol
100 kcal / mol
180 kcal / mol
SOLUTION
100 kcal / mol
71 The sample cells in should be
Only two surfaces to be polished
All surfaces should be polished
No polished surfaces should be present
None of the above
SOLUTION
All surfaces should be polished
72 Self quenching occurs when
Low concentration is present
High concentration is present
Medium concentration is present
None of the above
SOLUTION
High concentration is present
73 Commercial ethanol contains
Phenanthracene
Anthracene
Benzene
Terpenes
SOLUTION
Benzene
74 Which of the following is a far UV region
Above 200 mμ
Below 200 mμ
200 mμ
400 mμ
SOLUTION
Below 200 mμ
75 Which of the following is most suitable light source for UV – Visible spectroscopy
Hydrogen lamp
Carbon arc lamp source
Nernst glower
Radio frequency source
SOLUTION
Hydrogen lamp
76 Calculate the absorption maximum for the compound given
263mμ
273mµ
283mμ
293mμ
SOLUTION
273mµ
77 Primary filter absorbs
Visible & transmits IR radiation
UV & transmits IR radiation
Visible & transmits UV radiation
IR & transmits visible radiation
SOLUTION
Visible & transmits UV radiation
78 n⟶ σ* transition takes places in which type of compounds
Saturated compounds
Unsaturated compounds with one hetero atom
Saturated compounds with one hetero atom
Unsaturated compounds
SOLUTION
Saturated compounds with one hetero atom
79 In Single beam flourometer, the primary filter is kept at which angle to the secondary filter
360°
180°
60°
90°
SOLUTION
90°
80 The phenomenon of emission of light radiation by substance, when excitation occurs is called as
Fluorescence
Luminescence
Phosphorescence
Absorption
SOLUTION
Luminescence
81 If the wavelength of emitted radiation is equal to the absorbed radiation is called as
Anti-stokes fluorescence
Resonance fluorescence
Stokes fluorescence
Raleigh fluorescence
SOLUTION
Resonance fluorescence
82 If the wavelength of emitted radiation is equal to the absorbed radiation is called as
Anti-stokes fluorescence
Resonance fluorescence
Stokes fluorescence
Raleigh fluorescence
SOLUTION
Resonance fluorescence
SOLUTION
A & B are correct
83 Which of the following groups enhance fluorescence intensity
Electron donating groups
Electron negativity groups
Electron with drawing groups
Hydrogen bonding groups
SOLUTION
Electron donating groups
84 Removal of conjugation and in polarity of solvent causes
Hyperchromic shift
Hypsochromic shift
Bathochromic shift
Hypochromic shift
SOLUTION
Hypsochromic shift
85 Which of the following expression is related to Lambert’s law
dI/dx = kI
dI/dx = -kI
-dx/dI = kI
-dI/dx = kI
SOLUTION
-dI/dx = kI
86 Calculate the absorption the maximum in the UV spectrum of
232mμ
220mμ
221mμ
262 mμ
SOLUTION
232mμ
87 What is the other name for UV-Visible Spectroscopy
Electromagnetic radiation
Electromagnetic spectroscopy
Electronic spectroscopy
Electrochemical spectroscopy
SOLUTION
Electronic spectroscopy
88 Which of the following detectors in Fluorometry
Golay cells
Photovoltaic cells
Photo tubes
Photomultiplier tubes
SOLUTION
Photomultiplier tubes
89 The wavelength of absorbed radiation is called as
Phosphorescence
Fluorescence
Emission wavelength
Excitation wavelength
SOLUTION
Excitation wavelength
90 Which of the following are the examples for electron donating groups
—COON
-NO2
-OH
—CH3
SOLUTION
-OH
91 Which of the following functional groups does not undergo n⟶σ* transition
Alcohols
Esters
Ethers
Ketones
SOLUTION
Esters
92 Which of the following gas should not be present when fluorescence phenomenon is conducted
H2
He
O2
N2
SOLUTION
O2
93 Isomer of benzene which is yellow in color
Flavaonoids
Fulvene
Terpenes
Dienes
SOLUTION
Fulvene
94 Which radiation source has electrode in its construction
Tungsten lamp
Hydrogen discharge lamp
Xenon Discharge Lamp
Mercury lamp
SOLUTION
Hydrogen discharge lamp
95 Calculate the Emax for this transition for 2.5×10-4M solution of a substance in a lcm length cell at λmax 245nm has absorbance 1.17
0.468 x 107cm2/mole
0.568 x 107cm2/mole
0.668 x 107cm2/mole
0.768 x 107cm2/mole
SOLUTION
0.468 x 107cm2/mole
96In Spectroflourometer the filter in double beam flourometer is replaced by
Monochromators
Gratings
Prisms
Detectors
SOLUTION
Monochromators
97 Most commonly used substance to perform Fluorometry is
Quinine
Squill
Cinchona
Nux vomica
SOLUTION
Quinine
98 How much range wavelength is transmit by silicate glass
100 nm to 200 nm
200nm to 300 nm
300 nm to 350 nm
10nm to 40 nm
SOLUTION
300 nm to 350 nm
99 In quantitative T.L.C radioactive material can be studied by
Visual comparison
Gravimetry
Densitometer
Ginger counter
SOLUTION
Ginger counter
100 The wavelength of a radiation can be expressed in terms of
Frequency or energy in kcal/mol
Wave number or energy in kcal/ mol
Frequency or energy in k joule/ mol
Wave number or energy in k joule/mol
SOLUTION
Frequency or energy in kcal/mol
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